重庆市部分养殖场金黄色葡萄球菌耐药性分析及ESBL基因检测

doi:10.11843/j.issn.0366-6964.2018.05.023

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (5): 1074-1080.doi: 10.11843/j.issn.0366-6964.2018.05.023

重庆市部分养殖场金黄色葡萄球菌耐药性分析及ESBL基因检测

陈瑶, 丁红雷, 何英, 牟豪, 王立敏, 王豪举*

西南大学兽医传染病学实验室, 重庆 400715

收稿日期:2017-10-18 出版日期:2018-05-23 发布日期:2018-05-23
通讯作者: 王豪举,副教授,E-mail:kyc_whj@swu.edu.cn
作者简介:陈瑶(1992-),女,四川广安人,硕士生,主要从事预防兽医学研究,E-mail:374191461@qq.com
基金资助:
国家重点基础研究发展计划项目（2013CB12720）；重庆市社会事业与民生保障科技创新专项（cstc2015shmszx80033）

Antimicrobial Susceptibility Analysis and ESBL Gene Detection of Staphylococcus aureus Isolated at Animal Farms in Chongqing

CHEN Yao, DING Hong-lei, HE Ying, MU Hao, WANG Li-min, WANG Hao-ju*

Laboratory of Veterinary Lemology, Southwest University, Chongqing 400715, China

Received:2017-10-18 Online:2018-05-23 Published:2018-05-23

摘要/Abstract

摘要：

为了解重庆市部分养殖场动物源性金黄色葡萄球菌的耐药及携带超广谱β-内酰胺酶（extended-spectrum β-lactamases，ESBLs）基因情况，从重庆部分养殖场采集共1 371份样品，经前处理、增菌，划线接种，挑取疑似菌落，PCR扩增金黄色葡萄球菌特异性nuc基因，对分离鉴定的金黄色葡萄球菌进行药物敏感性试验，并检测菌株中携带的ESBL基因。结果显示：从1 371份样品中分离到89株金黄色葡萄球菌，包括25株猪源菌株、32株鸡源菌株、6株牛源菌株、10株羊源菌株和16株兔源菌株。89株菌对青霉素类药物耐药最为严重，其次为四环素类、大环内酯类和喹诺酮类。除2株兔源菌株外，其余分离菌均为多重耐药，耐药数量从2种到21种不等，猪源菌株的多重耐药最为严重。ESBL基因检测结果显示共有86株细菌携带bla_TEM-1a基因，携带率高达96.6%。分离的金黄色葡萄球菌菌株耐药严重，且广泛携带TEM-1a型ESBL基因。

Abstract:

This paper aims at understanding the information about the antimicrobial resistance and the existence of extended-spectrum β-lactamases (ESBL) genes in livestock-associated Staphylococcus aureus (S. aureus) at some animal farms of Chongqing. One thousand three hundred and seventy-one samples were collected from animal farms. After pretreating, enriching and culturing. The S. aureus specific gene nuc was amplified from the suspected colonies, and the antimicrobial susceptibility of the identified S. aureus was tested. Meanwhile, the ESBL genes were amplified. Results were as follows:Eighty-nine S. aureus clinical strains were isolated and identified from 1 371 samples, including 25 pig-originated strains, 32 chicken-originated strains, 6 dairy cattle-originated strains, 10 goat-originated strains, and 16 rabbit-originated strains. The isolates were most resistant to penicillins, and also more resistant to tetracyclines, macrolides, and quinolones. Except for 2 rabbit-originated strains, other isolates exhibited varying degrees of multi-drug resistance, defined as resistance to 2 to 21 antimicrobials. The most prevalent multi-drug resistant strains were pig-originated. The ESBL genes detection result showed that 86 (96.6%) organisms contained bla_TEM-1a. The S. aureus isolates were resistant to tested antimicrobials seriously, and most of them carried TEM-1a type ESBL.

中图分类号:

陈瑶, 丁红雷, 何英, 牟豪, 王立敏, 王豪举. 重庆市部分养殖场金黄色葡萄球菌耐药性分析及ESBL基因检测[J]. 畜牧兽医学报, 2018, 49(5): 1074-1080.

CHEN Yao, DING Hong-lei, HE Ying, MU Hao, WANG Li-min, WANG Hao-ju. Antimicrobial Susceptibility Analysis and ESBL Gene Detection of Staphylococcus aureus Isolated at Animal Farms in Chongqing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1074-1080.

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重庆市部分养殖场金黄色葡萄球菌耐药性分析及ESBL基因检测

Antimicrobial Susceptibility Analysis and ESBL Gene Detection of Staphylococcus aureus Isolated at Animal Farms in Chongqing

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